Complex organic molecules with several functional groups can require different and specific tritide reactants. The labelling will be at a predictable position with medium to high specific activity. The following scheme shows the huge variety of reagents available, which all have Lithium tritide (LiT) as their precursor. In order to achieve the highest specific activity possible, the reagents are always freshly prepared in our laboratory prior to use in the labelling reaction.
Various applications of these tritiated reducing agents are illustrated in the following schemes. As indicated, the specific activities achieved usually range between 10 to 29 Ci/mmol per tritium label. This is an excellent method for introducing a tritium label whenever high specific activity is required.
Reduction with Lithium Aluminum Tritide
Reduction with Lithium Triethylborotritide
Reduction with Lithium borotritide
Reductive Amination using Sodium Triacetoxyborohydride